Large-scale live imaging of adult neural stem cells in their endogenous niche

Nicolas Dray (1) , Sébastien Bedu (1) , Nelly Vuillemin (2) , Alessandro Alunni (1) , Marion Coolen (1) , Monika Krecsmarik (1) , Willy Supatto (2) , Emmanuel Beaurepaire (2) , Laure Bally-Cuif (1)

1 NeuroPSI - Institut des Neurosciences Paris-Saclay
2 LOB - Laboratoire d'Optique et Biosciences

Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.

Domains: Optics [physics.optics]; Imaging; Neurobiology

Nicolas Dray, Sébastien Bedu, Nelly Vuillemin, Alessandro Alunni, Marion Coolen, et al.. Large-scale live imaging of adult neural stem cells in their endogenous niche. Development (Cambridge, England), 2015, 142 (20), pp.3592-3600. ⟨10.1242/dev.123018⟩. ⟨hal-01221855⟩