Polarization-resolved Second Harmonic Imaging of collagen organization in connective tissues - Application to biomechanics

Claire Teulon (1) , Gaël Latour (1) , Ivan Gusachenko (1) , Guillaume Ducourthial (1) , Stéphane Bancelin (1) , Christelle Bonod-Bidaud (2) , Florence Ruggiero (2) , Jean-Sébastien Affagard (3) , Barbara Lynch (3) , Aurélie Benoit (3) , Jean-Marc Allain (3) , Marie-Claire Schanne-Klein (1)

1 LOB - Laboratoire d'Optique et Biosciences
2 IGFL - Institut de Génomique Fonctionnelle de Lyon
3 LMS - Laboratoire de mécanique des solides

Polarization-resolved SHG microscopy enables measurements of the main orientation and the angular dispersion of collagen fibrils within the focal volume. Results are presented in cornea, tendon and skin, eventually as function of mechanical stress.

Domains: Biomechanics [physics.med-ph]; Tissues and Organs [q-bio.TO]; Imaging

Claire Teulon, Gaël Latour, Ivan Gusachenko, Guillaume Ducourthial, Stéphane Bancelin, et al.. Polarization-resolved Second Harmonic Imaging of collagen organization in connective tissues - Application to biomechanics. Novel Techniques in Microscopy, 2017, San Diego, United States. pp.NW3C.2, ⟨10.1364/NTM.2017.NW3C.2⟩. ⟨hal-01761415⟩